Dynamic competition between SARS-CoV-2 NSP1 and mRNA on the human ribosome inhibits translation initiation

Significance SARS-CoV-2 is the causative agent of COVID-19 pandemic. A molecular framework for how virus manipulates host cellular machinery to facilitate infection needed. Here, we integrate biochemical and single-molecule strategies reveal insight into NSP1 from inhibits translation initiation. directly binds small (40S) subunit human ribosome, which modulated by initiation factors. Further, mRNA compete with each other bind ribosome. Our findings suggest that presence on ribosomal prevents proper accommodation mRNA. How this competition disrupts many steps an important target future studies.